ELISA (Enzyme Linked Immunosorbent Assay)
Enzyme-linked immunosorbent assays (ELISA) functions on principles of specific interactions between antibody and antigen. Specific antibodies bind the target antigen, peptides or proteins and a detection system indicates the presence and quantity of antigen bound to the antibody.
In this technique, an antigen is immobilized to a solid surface and then complexed with an antibody that is linked to an enzyme to facilitate detection of a substrate. The quantification of bound product is done by optical densitometry of the substrate.