ELISA (Enzyme Linked Immunosorbent Assay)




Enzyme-linked immunosorbent assays (ELISA) functions on principles of specific interactions between antibody and antigen. Specific antibodies bind the target antigen, peptides or proteins and a detection system indicates the presence and quantity of antigen bound to the antibody.

In this technique, an antigen is immobilized to a solid surface and then complexed with an antibody that is linked to an enzyme to facilitate detection of a substrate. The quantification of bound product is done by optical densitometry of the substrate.


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